An archaeal orthologue of the universal protein Kae1 is an iron metalloprotein which exhibits atypical DNA-binding properties and apurinic-endonuclease activity in vitro
Identifieur interne : 000186 ( France/Analysis ); précédent : 000185; suivant : 000187An archaeal orthologue of the universal protein Kae1 is an iron metalloprotein which exhibits atypical DNA-binding properties and apurinic-endonuclease activity in vitro
Auteurs : Arnaud Hecker [France] ; Nicolas Leulliot [France] ; Danièle Gadelle [France] ; Marc Graille [France] ; Anthony Justome [France] ; Pierre Dorlet [France] ; Céline Brochier [France] ; Sophie Quevillon-Cheruel [France] ; Eric Le Cam [France] ; Herman Van Tilbeurgh [France] ; Patrick Forterre [France]Source :
- Nucleic Acids Research [ 0305-1048 ] ; 2007.
Abstract
The Kae1 (Kinase-associated endopeptidase 1) protein is a member of the recently identified transcription complex EKC and telomeres maintenance complex KEOPS in yeast. Kae1 homologues are encoded by all sequenced genomes in the three domains of life. Although annotated as putative endopeptidases, the actual functions of these universal proteins are unknown. Here we show that the purified Kae1 protein (Pa-Kae1) from Pyrococcus abyssi is an iron-protein with a novel type of ATP-binding site. Surprisingly, this protein did not exhibit endopeptidase activity in vitro but binds cooperatively to single and double-stranded DNA and induces unusual DNA conformational change. Furthermore, Pa-Kae1 exhibits a class I apurinic (AP)-endonuclease activity (AP-lyase). Both DNA binding and AP-endonuclease activity are inhibited by ATP. Kae1 is thus a novel and atypical universal DNA interacting protein whose importance could rival those of RecA (RadA/Rad51) in the maintenance of genome integrity in all living cells.
Url:
DOI: 10.1093/nar/gkm554
Affiliations:
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Paris-Sud, IFR115, UMR8621-CNRS, 91405 Orsay, France, Institut de Biochimie et de Biophysique Moléculaire et Cellulaire, Univ. Paris-Sud, IFR115, UMR8619-CNRS, 91405 Orsay, France, Institut Gustave Roussy, Interactions Moléculaires et Cancer, UMR8126-CNRS, 94805 Villejuif Cedex, France, Institut de Chimie Moléculaire et des Matériaux, Univ. Paris-Sud, UMR8182-CNRS, 91405 Orsay, France, Institut de Biologie Structurale et de Microbiologie, Laboratoire de Chimie Bactérienne, UPR9043-CNRS, 13402 Marseille Cedex 20, France, Université de Provence - Aix-Marseille I, 13331 Marseille Cedex 3, France and Institut Pasteur, Unité Biologie Moléculaire du Gène chez les Extrêmophiles, 25 rue du Dr Roux, 75724 Paris Cedex 15</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Leulliot, Nicolas" sort="Leulliot, Nicolas" uniqKey="Leulliot N" first="Nicolas" last="Leulliot">Nicolas Leulliot</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Institut de Génétique et Microbiologie, Univ. Paris-Sud, IFR115, UMR8621-CNRS, 91405 Orsay, France, Institut de Biochimie et de Biophysique Moléculaire et Cellulaire, Univ. Paris-Sud, IFR115, UMR8619-CNRS, 91405 Orsay, France, Institut Gustave Roussy, Interactions Moléculaires et Cancer, UMR8126-CNRS, 94805 Villejuif Cedex, France, Institut de Chimie Moléculaire et des Matériaux, Univ. Paris-Sud, UMR8182-CNRS, 91405 Orsay, France, Institut de Biologie Structurale et de Microbiologie, Laboratoire de Chimie Bactérienne, UPR9043-CNRS, 13402 Marseille Cedex 20, France, Université de Provence - Aix-Marseille I, 13331 Marseille Cedex 3, France and Institut Pasteur, Unité Biologie Moléculaire du Gène chez les Extrêmophiles, 25 rue du Dr Roux, 75724 Paris Cedex 15</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Gadelle, Daniele" sort="Gadelle, Daniele" uniqKey="Gadelle D" first="Danièle" last="Gadelle">Danièle Gadelle</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Institut de Génétique et Microbiologie, Univ. Paris-Sud, IFR115, UMR8621-CNRS, 91405 Orsay, France, Institut de Biochimie et de Biophysique Moléculaire et Cellulaire, Univ. Paris-Sud, IFR115, UMR8619-CNRS, 91405 Orsay, France, Institut Gustave Roussy, Interactions Moléculaires et Cancer, UMR8126-CNRS, 94805 Villejuif Cedex, France, Institut de Chimie Moléculaire et des Matériaux, Univ. Paris-Sud, UMR8182-CNRS, 91405 Orsay, France, Institut de Biologie Structurale et de Microbiologie, Laboratoire de Chimie Bactérienne, UPR9043-CNRS, 13402 Marseille Cedex 20, France, Université de Provence - Aix-Marseille I, 13331 Marseille Cedex 3, France and Institut Pasteur, Unité Biologie Moléculaire du Gène chez les Extrêmophiles, 25 rue du Dr Roux, 75724 Paris Cedex 15</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Graille, Marc" sort="Graille, Marc" uniqKey="Graille M" first="Marc" last="Graille">Marc Graille</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Institut de Génétique et Microbiologie, Univ. Paris-Sud, IFR115, UMR8621-CNRS, 91405 Orsay, France, Institut de Biochimie et de Biophysique Moléculaire et Cellulaire, Univ. Paris-Sud, IFR115, UMR8619-CNRS, 91405 Orsay, France, Institut Gustave Roussy, Interactions Moléculaires et Cancer, UMR8126-CNRS, 94805 Villejuif Cedex, France, Institut de Chimie Moléculaire et des Matériaux, Univ. Paris-Sud, UMR8182-CNRS, 91405 Orsay, France, Institut de Biologie Structurale et de Microbiologie, Laboratoire de Chimie Bactérienne, UPR9043-CNRS, 13402 Marseille Cedex 20, France, Université de Provence - Aix-Marseille I, 13331 Marseille Cedex 3, France and Institut Pasteur, Unité Biologie Moléculaire du Gène chez les Extrêmophiles, 25 rue du Dr Roux, 75724 Paris Cedex 15</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Justome, Anthony" sort="Justome, Anthony" uniqKey="Justome A" first="Anthony" last="Justome">Anthony Justome</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Institut de Génétique et Microbiologie, Univ. Paris-Sud, IFR115, UMR8621-CNRS, 91405 Orsay, France, Institut de Biochimie et de Biophysique Moléculaire et Cellulaire, Univ. Paris-Sud, IFR115, UMR8619-CNRS, 91405 Orsay, France, Institut Gustave Roussy, Interactions Moléculaires et Cancer, UMR8126-CNRS, 94805 Villejuif Cedex, France, Institut de Chimie Moléculaire et des Matériaux, Univ. Paris-Sud, UMR8182-CNRS, 91405 Orsay, France, Institut de Biologie Structurale et de Microbiologie, Laboratoire de Chimie Bactérienne, UPR9043-CNRS, 13402 Marseille Cedex 20, France, Université de Provence - Aix-Marseille I, 13331 Marseille Cedex 3, France and Institut Pasteur, Unité Biologie Moléculaire du Gène chez les Extrêmophiles, 25 rue du Dr Roux, 75724 Paris Cedex 15</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Dorlet, Pierre" sort="Dorlet, Pierre" uniqKey="Dorlet P" first="Pierre" last="Dorlet">Pierre Dorlet</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Institut de Génétique et Microbiologie, Univ. Paris-Sud, IFR115, UMR8621-CNRS, 91405 Orsay, France, Institut de Biochimie et de Biophysique Moléculaire et Cellulaire, Univ. Paris-Sud, IFR115, UMR8619-CNRS, 91405 Orsay, France, Institut Gustave Roussy, Interactions Moléculaires et Cancer, UMR8126-CNRS, 94805 Villejuif Cedex, France, Institut de Chimie Moléculaire et des Matériaux, Univ. Paris-Sud, UMR8182-CNRS, 91405 Orsay, France, Institut de Biologie Structurale et de Microbiologie, Laboratoire de Chimie Bactérienne, UPR9043-CNRS, 13402 Marseille Cedex 20, France, Université de Provence - Aix-Marseille I, 13331 Marseille Cedex 3, France and Institut Pasteur, Unité Biologie Moléculaire du Gène chez les Extrêmophiles, 25 rue du Dr Roux, 75724 Paris Cedex 15</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Brochier, Celine" sort="Brochier, Celine" uniqKey="Brochier C" first="Céline" last="Brochier">Céline Brochier</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Institut de Génétique et Microbiologie, Univ. Paris-Sud, IFR115, UMR8621-CNRS, 91405 Orsay, France, Institut de Biochimie et de Biophysique Moléculaire et Cellulaire, Univ. Paris-Sud, IFR115, UMR8619-CNRS, 91405 Orsay, France, Institut Gustave Roussy, Interactions Moléculaires et Cancer, UMR8126-CNRS, 94805 Villejuif Cedex, France, Institut de Chimie Moléculaire et des Matériaux, Univ. Paris-Sud, UMR8182-CNRS, 91405 Orsay, France, Institut de Biologie Structurale et de Microbiologie, Laboratoire de Chimie Bactérienne, UPR9043-CNRS, 13402 Marseille Cedex 20, France, Université de Provence - Aix-Marseille I, 13331 Marseille Cedex 3, France and Institut Pasteur, Unité Biologie Moléculaire du Gène chez les Extrêmophiles, 25 rue du Dr Roux, 75724 Paris Cedex 15</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Quevillon Cheruel, Sophie" sort="Quevillon Cheruel, Sophie" uniqKey="Quevillon Cheruel S" first="Sophie" last="Quevillon-Cheruel">Sophie Quevillon-Cheruel</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Institut de Génétique et Microbiologie, Univ. Paris-Sud, IFR115, UMR8621-CNRS, 91405 Orsay, France, Institut de Biochimie et de Biophysique Moléculaire et Cellulaire, Univ. Paris-Sud, IFR115, UMR8619-CNRS, 91405 Orsay, France, Institut Gustave Roussy, Interactions Moléculaires et Cancer, UMR8126-CNRS, 94805 Villejuif Cedex, France, Institut de Chimie Moléculaire et des Matériaux, Univ. Paris-Sud, UMR8182-CNRS, 91405 Orsay, France, Institut de Biologie Structurale et de Microbiologie, Laboratoire de Chimie Bactérienne, UPR9043-CNRS, 13402 Marseille Cedex 20, France, Université de Provence - Aix-Marseille I, 13331 Marseille Cedex 3, France and Institut Pasteur, Unité Biologie Moléculaire du Gène chez les Extrêmophiles, 25 rue du Dr Roux, 75724 Paris Cedex 15</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Le Cam, Eric" sort="Le Cam, Eric" uniqKey="Le Cam E" first="Eric" last="Le Cam">Eric Le Cam</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Institut de Génétique et Microbiologie, Univ. Paris-Sud, IFR115, UMR8621-CNRS, 91405 Orsay, France, Institut de Biochimie et de Biophysique Moléculaire et Cellulaire, Univ. Paris-Sud, IFR115, UMR8619-CNRS, 91405 Orsay, France, Institut Gustave Roussy, Interactions Moléculaires et Cancer, UMR8126-CNRS, 94805 Villejuif Cedex, France, Institut de Chimie Moléculaire et des Matériaux, Univ. Paris-Sud, UMR8182-CNRS, 91405 Orsay, France, Institut de Biologie Structurale et de Microbiologie, Laboratoire de Chimie Bactérienne, UPR9043-CNRS, 13402 Marseille Cedex 20, France, Université de Provence - Aix-Marseille I, 13331 Marseille Cedex 3, France and Institut Pasteur, Unité Biologie Moléculaire du Gène chez les Extrêmophiles, 25 rue du Dr Roux, 75724 Paris Cedex 15</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Van Tilbeurgh, Herman" sort="Van Tilbeurgh, Herman" uniqKey="Van Tilbeurgh H" first="Herman" last="Van Tilbeurgh">Herman Van Tilbeurgh</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Institut de Génétique et Microbiologie, Univ. Paris-Sud, IFR115, UMR8621-CNRS, 91405 Orsay, France, Institut de Biochimie et de Biophysique Moléculaire et Cellulaire, Univ. Paris-Sud, IFR115, UMR8619-CNRS, 91405 Orsay, France, Institut Gustave Roussy, Interactions Moléculaires et Cancer, UMR8126-CNRS, 94805 Villejuif Cedex, France, Institut de Chimie Moléculaire et des Matériaux, Univ. Paris-Sud, UMR8182-CNRS, 91405 Orsay, France, Institut de Biologie Structurale et de Microbiologie, Laboratoire de Chimie Bactérienne, UPR9043-CNRS, 13402 Marseille Cedex 20, France, Université de Provence - Aix-Marseille I, 13331 Marseille Cedex 3, France and Institut Pasteur, Unité Biologie Moléculaire du Gène chez les Extrêmophiles, 25 rue du Dr Roux, 75724 Paris Cedex 15</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Forterre, Patrick" sort="Forterre, Patrick" uniqKey="Forterre P" first="Patrick" last="Forterre">Patrick Forterre</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Institut de Génétique et Microbiologie, Univ. Paris-Sud, IFR115, UMR8621-CNRS, 91405 Orsay, France, Institut de Biochimie et de Biophysique Moléculaire et Cellulaire, Univ. Paris-Sud, IFR115, UMR8619-CNRS, 91405 Orsay, France, Institut Gustave Roussy, Interactions Moléculaires et Cancer, UMR8126-CNRS, 94805 Villejuif Cedex, France, Institut de Chimie Moléculaire et des Matériaux, Univ. Paris-Sud, UMR8182-CNRS, 91405 Orsay, France, Institut de Biologie Structurale et de Microbiologie, Laboratoire de Chimie Bactérienne, UPR9043-CNRS, 13402 Marseille Cedex 20, France, Université de Provence - Aix-Marseille I, 13331 Marseille Cedex 3, France and Institut Pasteur, Unité Biologie Moléculaire du Gène chez les Extrêmophiles, 25 rue du Dr Roux, 75724 Paris Cedex 15</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">France</country></affiliation><affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Nucleic Acids Research</title><idno type="ISSN">0305-1048</idno><idno type="eISSN">1362-4962</idno><imprint><publisher>Oxford University Press</publisher><date type="published">2007</date><date type="e-published">2007</date><biblScope unit="vol">35</biblScope><biblScope unit="issue">18</biblScope><biblScope unit="page" from="6042">6042</biblScope><biblScope unit="page" to="6051">6051</biblScope></imprint><idno type="ISSN">0305-1048</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0305-1048</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">The Kae1 (Kinase-associated endopeptidase 1) protein is a member of the recently identified transcription complex EKC and telomeres maintenance complex KEOPS in yeast. Kae1 homologues are encoded by all sequenced genomes in the three domains of life. Although annotated as putative endopeptidases, the actual functions of these universal proteins are unknown. Here we show that the purified Kae1 protein (Pa-Kae1) from Pyrococcus abyssi is an iron-protein with a novel type of ATP-binding site. Surprisingly, this protein did not exhibit endopeptidase activity in vitro but binds cooperatively to single and double-stranded DNA and induces unusual DNA conformational change. Furthermore, Pa-Kae1 exhibits a class I apurinic (AP)-endonuclease activity (AP-lyase). Both DNA binding and AP-endonuclease activity are inhibited by ATP. Kae1 is thus a novel and atypical universal DNA interacting protein whose importance could rival those of RecA (RadA/Rad51) in the maintenance of genome integrity in all living cells.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Paris</li></settlement></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Hecker, Arnaud" sort="Hecker, Arnaud" uniqKey="Hecker A" first="Arnaud" last="Hecker">Arnaud Hecker</name></region><name sortKey="Brochier, Celine" sort="Brochier, Celine" uniqKey="Brochier C" first="Céline" last="Brochier">Céline Brochier</name><name sortKey="Dorlet, Pierre" sort="Dorlet, Pierre" uniqKey="Dorlet P" first="Pierre" last="Dorlet">Pierre Dorlet</name><name sortKey="Forterre, Patrick" sort="Forterre, Patrick" uniqKey="Forterre P" first="Patrick" last="Forterre">Patrick Forterre</name><name sortKey="Forterre, Patrick" sort="Forterre, Patrick" uniqKey="Forterre P" first="Patrick" last="Forterre">Patrick Forterre</name><name sortKey="Gadelle, Daniele" sort="Gadelle, Daniele" uniqKey="Gadelle D" first="Danièle" last="Gadelle">Danièle Gadelle</name><name sortKey="Graille, Marc" sort="Graille, Marc" uniqKey="Graille M" first="Marc" last="Graille">Marc Graille</name><name sortKey="Justome, Anthony" sort="Justome, Anthony" uniqKey="Justome A" first="Anthony" last="Justome">Anthony Justome</name><name sortKey="Le Cam, Eric" sort="Le Cam, Eric" uniqKey="Le Cam E" first="Eric" last="Le Cam">Eric Le Cam</name><name sortKey="Leulliot, Nicolas" sort="Leulliot, Nicolas" uniqKey="Leulliot N" first="Nicolas" last="Leulliot">Nicolas Leulliot</name><name sortKey="Quevillon Cheruel, Sophie" sort="Quevillon Cheruel, Sophie" uniqKey="Quevillon Cheruel S" first="Sophie" last="Quevillon-Cheruel">Sophie Quevillon-Cheruel</name><name sortKey="Van Tilbeurgh, Herman" sort="Van Tilbeurgh, Herman" uniqKey="Van Tilbeurgh H" first="Herman" last="Van Tilbeurgh">Herman Van Tilbeurgh</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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